HISTOPATHOLOGICAL EFFECTS OF SHILAJIT ON METHOTREXATE INDUCED TESTICULAR DAMAGE IN RATS

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OBJECTIVE: Methotrexate, only using form of antimetabolite group, has been used for breast, bladder and testicular cancer treatment. In this study, we aimed to examine histopathologicly the effects of Shilajit (PS) on Methotrexate (MTX) induced testis damage.MATERIALS AND METHODS: Twenty four Spraque Dawley male rats were divided up four groups. Control group was not treated. Group MTX was exposed to a single dose intraperitoneal (i.p.) 20 mg/kg MTX, Group MTX+PS was exposed to 100 mg/kg PS in 2ml/kg saline by gava-ge before 15 minutes and then a single dose i.p. 20 mg/kg MTX. After first dosage of PS, it was given at the same dosage two times at the every other day during the expe-riment (7days). Group PS was exposed to only 100 mg/kg PS in 2ml/kg saline by gavage. At the end of experiement (7th day), all animals were sacrificed and left testises were taken. Testicular tissue samples were evaluated as histo-patologically on the light microscope.RESULTS: When all groups compared with each other according to testicular and body weights, there was no statistically significant difference between all groups (p 0.05). At the histopathological evaluation; the diamater and epithelial thickness of seminiforus tubules signifi-cantly decreased in Group MTX (p 0.04) and reduction was less in Group MTX+ PS compared with Group MTX (p 0.04). At the morfological evaluation; immature tu-bular epithelial cells, decreasing of sperm amount in the lumen, irregularities in the tubule epithelium and in the basal membrane, and edema in the interstitial space were observed in Group MTX. İt was observed no patho-logical changes in the seminiforus tubules except a few ones that represented irregularities and immature tubu-lar epithelial cells in Group MTX+ PS.CONCLUSION: Our results showed that (MTX) might give rise to testicular damage and the treatment of PS may prevent this damage. As a result, PS may be useful for the prevention of testicular damage after MTX treatment.

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Methotrexate , Shilajit , Rat , Testis

Kocatepe Medical Journal

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Kocatepe Medical Journal