Author/Authors
serdaroğlu kaşikçi, emel üsküdar üniversitesi - mühendislik ve doğa bilimleri fakültesi, nöropsikofarmakoloji uygulama ve araştırma merkezi - moleküler biyoloji ve genetik bölümü, İstanbul, turkey , çevreli, burcu üsküdar üniversitesi - nöropsikofarmakoloji uygulama ve araştırma merkezi, İstanbul, Turkey , cengiz, kaan üsküdar üniversitesi - mühendislik ve doğa bilimleri fakültesi - moleküler biyoloji ve genetik bölümü, İstanbul, Turkey , coşkun, uğur üsküdar üniversitesi - mühendislik ve doğa bilimleri fakültesi - moleküler biyoloji ve genetik bölümü, İstanbul, Turkey , gözler, tayfun üsküdar üniversitesi - nöropsikofarmakoloji uygulama ve araştırma merkezi, İstanbul, turkey , tanhan, berfin üsküdar üniversitesi - mühendislik ve doğa bilimleri fakültesi - moleküler biyoloji ve genetik bölümü, İstanbul, Turkey , konuk, muhsin üsküdar üniversitesi - mühendislik ve doğa bilimleri fakültesi - moleküler biyoloji ve genetik bölümü, İstanbul, turkey
Title Of Article
EFFECT OF COMBINED IMPLEMENTATION HESPERIDIN AND VALPROIC ACID ON OXIDANT-ANTIOXIDANT DEFENSE IN A PENTYLENETETRAZOL INDUCED EPILEPSY MODEL
شماره ركورد
44952
Abstract
Epilepsy is a complex neurological condition associated with significant changes in psychological and emotional parameters affecting approximately 1% of the population worldwide. Oxidative stress is a common pathway used to explain neuronal damage in many neurodegenerative disorders. Hesperidin, a bioflavonoid, has been reported to have neuroprotective, anti-inflammatory, analgesic, antibacterial, antihypercholesterolemic and anticancer properties. In this study, it was aimed to investigate the effects of combined use of a valproic acid, an antiepileptic drug, with hesperidin on oxidant-antioxidant defense system both in brain and liver tissue specimens. For this purpose, a total of 48, 3-months old, and 200-250 gram male Wistar-Albino rats were divided into 6 groups. Group 1 was the Control. Group 2: pentylenetetrazole (60 mg / kg intraperitoneal), Group 3: hesperidin (100 mg / kg oral), Group 4: pentylenetetrazole + hesperidin, Group 5: pentylenetetrazole + valproic acid (400 mg / kg ip), Group 6: pentylenetetrazole + valproic acid + hesperidin. The results were subjected to statistical analysis by employing SPSS software and p≤0.05 accepted as significant value. Chemical agents were given to all groups outside the control group at 1-7-14-28 days. Glutathione, malondialdehyde levels and superoxide dismutase activity were determined in the brain and liver tissue homogenates obtained at the end of the experiment. The results showed that while glutathione levels and superoxide dismutase activity decreased, malondialdehyde levels increased significantly in pentylenetetrazole group. In the combination of valproic acid and hesperidin increased brain and liver glutathione levels significantly and decreased malondialdehyde levels in the epilepsy model produced by pentylenetetrazole. It was also observed that hesperidin significantly increased superoxide dismutase activity in brain tissue samples when compared to pentylenetetrazole and pentylenetetrazole + valproic acid groups when it was given alone or together with valproic acid. In liver tissue, hesperidin increased superoxide dismutase activity significantly compared to control group, and superoxide dismutase activity increased significantly in brain and liver specimens compared to both pentylenetetrazole group and hesperidin group. Our results demonstrate that hesperidin may increase antioxidant activity when used in combination with antiepileptic drugs and may reduce the potential side effects of valproic acid alone.
From Page
106
NaturalLanguageKeyword
Epilepsy , Hesperidin , Valproic acid , Oxidant , antioxidant defence system
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
120
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
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