Title :
Determination of chloroplast DNA in a cultured soybean line using a QCM biosensor
Author :
Towery, Robert B. ; Fawcett, Newton C. ; Evans, Jeffrey A.
Author_Institution :
Dept. of Chem., Houston Baptist Univ., TX, USA
Abstract :
The percent of chloroplast DNA (chl-DNA) in total soybean DNA from the glycine max [L.] Merr. v Corsoy cell line was redetermined using DNA-hybridization biosensors made from the quartz crystal microbalance. The percent of chl-DNA was found to be 30.6% ± 2.0%, consistent with the value found earlier for cells grown either photoautotrophically or photomixotrophically. The method avoids radio or other types of molecular labels and is simple and relatively rapid when compared to conventional hybridization assays.
Keywords :
DNA; biosensors; botany; cellular biophysics; microbalances; DNA-hybridization biosensors; QCM biosensor; cell line; chl-DNA; chloroplast DNA; cultured soybean line; glycine max; molecular labels; photoautotrophically grown cells; photomixotrophically grown cells; quartz crystal microbalance; soybean DNA; Amino acids; Biomembranes; Biosensors; Cells (biology); Chemistry; DNA; Genomics; Nanobioscience; Probes; Sequences; Biosensor; QCM; chl-DNA; chloroplast DNA; hybridization; quartz crystal microbalance;
Journal_Title :
Sensors Journal, IEEE
DOI :
10.1109/JSEN.2004.830956
