DocumentCode
1026754
Title
Determination of chloroplast DNA in a cultured soybean line using a QCM biosensor
Author
Towery, Robert B. ; Fawcett, Newton C. ; Evans, Jeffrey A.
Author_Institution
Dept. of Chem., Houston Baptist Univ., TX, USA
Volume
4
Issue
4
fYear
2004
Firstpage
489
Lastpage
493
Abstract
The percent of chloroplast DNA (chl-DNA) in total soybean DNA from the glycine max [L.] Merr. v Corsoy cell line was redetermined using DNA-hybridization biosensors made from the quartz crystal microbalance. The percent of chl-DNA was found to be 30.6% ± 2.0%, consistent with the value found earlier for cells grown either photoautotrophically or photomixotrophically. The method avoids radio or other types of molecular labels and is simple and relatively rapid when compared to conventional hybridization assays.
Keywords
DNA; biosensors; botany; cellular biophysics; microbalances; DNA-hybridization biosensors; QCM biosensor; cell line; chl-DNA; chloroplast DNA; cultured soybean line; glycine max; molecular labels; photoautotrophically grown cells; photomixotrophically grown cells; quartz crystal microbalance; soybean DNA; Amino acids; Biomembranes; Biosensors; Cells (biology); Chemistry; DNA; Genomics; Nanobioscience; Probes; Sequences; Biosensor; QCM; chl-DNA; chloroplast DNA; hybridization; quartz crystal microbalance;
fLanguage
English
Journal_Title
Sensors Journal, IEEE
Publisher
ieee
ISSN
1530-437X
Type
jour
DOI
10.1109/JSEN.2004.830956
Filename
1310341
Link To Document