Surface Modification for Protein and DNA Immobilization onto GMR Biosensor

Author

Wang, Wei ; Wang, Yi ; Tu, Liang ; Klein, Todd ; Feng, Yinglong ; Wang, Jian-Ping

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Minnesota, Minneapolis, MN, USA

Volume

49

Issue

1

fYear

2013

fDate

Jan. 2013

Firstpage

296

Lastpage

299

Abstract

Giant magnetoresistance (GMR) biosensor with 20 nm SiO ₂ on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

Keywords

DNA; biosensors; chemical sensors; fluorescence spectroscopy; giant magnetoresistance; magnetic particles; magnetic sensors; molecular biophysics; nanoparticles; proteins; spectrochemical analysis; 3-aminopropyltriethoxy silane; APTES-Glu modification method; DNA immobilization; GMR biosensor; IL-6 antibody; IL-6 antigen; SiO₂; amine modified DNA oligonucleotide; complementary DNA oligonucleotide; deoxyribonucleic acid; fluorescence sandwich assay; functionalized surface; giant magnetoresistance biosensor; glutaraldehyde; interleukin-6 antibody; protein immobilization; size 20 nm; size 30 nm; streptavidin labeled magnetic nanoparticles; surface modification; terminal aldehyde groups; Biosensors; DNA; Immune system; Molecular biophysics; Proteins; Silicon; Surface treatment; Deoxyribonucleic acid (DNA); giant magnetoresistance (GMR) biosensor; immobilization; protein; surface modification;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2012.2224327

Filename

6392433