Title :
The arrival of genetic engineering
Author :
Arulmuthu, Eugene R. ; Williams, David J. ; Versteeg, Hendrik K.
Author_Institution :
Gene Therapy Consortium, UCL, London
Abstract :
The purpose of this article is to review the different strategies for the delivery of plasmid DNA (pDNA)-based gene therapy. Plasmids are extrachromosomal DNA capable of being transmitted from cell to cell. They are supercoiled, circular covalently closed (ccc) strands of DNA capable of replication independently of host DNA and ranging from 5 to 400 kilo base pairs (kb) in size, depending on the size of the therapeutic gene insert.
Keywords :
DNA; cancer; cellular biophysics; diseases; gene therapy; genetic engineering; genetics; lung; molecular biophysics; aerosol gene delivery; chronic respiratory diseases; cystic fibrosis; extrachromosomal DNA; genetic engineering; influenza; lung cancer; nonviral gene therapeutics; plasmid DNA-based gene therapy; Bioinformatics; Clinical trials; DNA; Diseases; Gene therapy; Genetic engineering; Genomics; Humans; Lungs; Medical treatment; Cystic Fibrosis; Drug Administration Routes; Gene Therapy; Gene Transfer Techniques; Humans; Magnetics; Metered Dose Inhalers; Nebulizers and Vaporizers; Peptide Nucleic Acids; Plasmids;
Journal_Title :
Engineering in Medicine and Biology Magazine, IEEE
DOI :
10.1109/MEMB.2008.931015
