Title :
Miniaturized Detection System for Fluorescence and Absorbance Measurements in Chromatographic Applications
Author :
Overmeire, Sara Van ; Ottevaere, Heidi ; Desmet, Gert ; Thienpont, Hugo
Author_Institution :
Vrije Univ. Brussel, Brussels
Abstract :
We present a microoptical detection unit for both laser-induced fluorescence and absorbance analysis on fused silica capillaries, which can be used for chromatographic applications. The detection system is designed by means of nonsequential ray tracing simulations and prototyped by means of deep proton writing. In a proof-of-concept demonstration, the microoptical unit is used for the detection of various concentrations of coumarin dyes. The detection limit (SNR=3.3) achieved measures 0.6 nM for fluorescence analysis and 12 muM for absorbance measurements in capillaries with an inner diameter of 150 mum.
Keywords :
chemical variables measurement; chromatography; dyes; fluorescence; measurement by laser beam; micro-optomechanical devices; microsensors; optical sensors; absorbance measurements; chromatographic applications; coumarin dye concentration detection; deep proton writing; detection limitation; fused silica capillaries; laser-induced fluorescence measurements; microoptical detection unit; miniaturized detection system; nonsequential ray tracing simulations; size 150 mum; Associate members; Chemical analysis; Fluorescence; Microfluidics; Optical devices; Protons; Ray tracing; Robustness; Silicon compounds; Virtual prototyping; Absorbance; chromatography; fluorescence; optics; plastics; rapid prototyping;
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
DOI :
10.1109/JSTQE.2007.912753
