DocumentCode :
108131
Title :
A 1024-Channel CMOS Microelectrode Array With 26,400 Electrodes for Recording and Stimulation of Electrogenic Cells In Vitro
Author :
Ballini, Marco ; Muller, Johannes ; Livi, Paolo ; Yihui Chen ; Frey, Urs ; Stettler, Alexander ; Shadmani, Amir ; Viswam, Vijay ; Lloyd Jones, Ian ; Jackel, David ; Radivojevic, Milos ; Lewandowska, Marta K. ; Wei Gong ; Fiscella, Michele ; Bakkum, Dougla
Author_Institution :
D-BSSE, ETH Zurich, Basel, Switzerland
Volume :
49
Issue :
11
fYear :
2014
fDate :
Nov. 2014
Firstpage :
2705
Lastpage :
2719
Abstract :
To advance our understanding of the functioning of neuronal ensembles, systems are needed to enable simultaneous recording from a large number of individual neurons at high spatiotemporal resolution and good signal-to-noise ratio. Moreover, stimulation capability is highly desirable for investigating, for example, plasticity and learning processes. Here, we present a microelectrode array (MEA) system on a single CMOS die for in vitro recording and stimulation. The system incorporates 26,400 platinum electrodes, fabricated by in-house post-processing, over a large sensing area (3.85 2.10 mm ) with sub-cellular spatial resolution (pitch of 17.5 μm). Owing to an area and power efficient implementation, we were able to integrate 1024 readout channels on chip to record extracellular signals from a user-specified selection of electrodes. These channels feature noise values of 2.4 μV in the action-potential band (300 Hz-10 kHz) and 5.4 μV in the local-field-potential band (1 Hz-300 Hz), and provide programmable gain (up to 78 dB) to accommodate various biological preparations. Amplified and filtered signals are digitized by 10 bit parallel single-slope ADCs at 20 kSamples/s. The system also includes 32 stimulation units, which can elicit neural spikes through either current or voltage pulses. The chip consumes only 75 mW in total, which obviates the need of active cooling even for sensitive cell cultures.
Keywords :
CMOS integrated circuits; analogue-digital conversion; array signal processing; bioelectric phenomena; biomedical electrodes; cellular biophysics; medical signal processing; microelectrodes; neural nets; 1024-channel CMOS microelectrode array; ADC; CMOS die; MEA system; amplified signals; bandwidth 1 Hz to 300 Hz; bandwidth 300 Hz to 10 kHz; electrogenic cells; extracellular signals; filtered signals; in vitro recording; in vitro stimulation; neural spikes; neuronal ensembles; power 75 mW; signal-to-noise ratio; spatiotemporal resolution; subcellular spatial resolution; voltage 2.4 muV; voltage 5.4 muV; Arrays; CMOS integrated circuits; Microelectrodes; Noise; Spatial resolution; Switches; Extracellular recording and stimulation; high channel count; low noise; low power; microelectrode array (MEA); multirate switched capacitor filter; neural interface; offset compensation; single-slope ADC; switch matrix;
fLanguage :
English
Journal_Title :
Solid-State Circuits, IEEE Journal of
Publisher :
ieee
ISSN :
0018-9200
Type :
jour
DOI :
10.1109/JSSC.2014.2359219
Filename :
6923484
Link To Document :
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