The Bio-Oscillator: A Circuit for Cell-Culture Assays

Author

Huertas, Gloria ; Maldonado, Andres ; Yufera, Alberto ; Rueda, Adoracion ; Huertas, Jose Luis

Author_Institution

Centro Nac. de Microelectron., Inst. de Microelectron. de Sevilla, Sevilla, Spain

Volume

62

Issue

2

fYear

2015

fDate

Feb. 2015

Firstpage

164

Lastpage

168

Abstract

A system for cell-culture real-time monitoring using an oscillation-based approach is proposed. The system transforms a cell culture under test into a suitable “biological” oscillator, without needing complex circuitry for excitation and measurement. The obtained oscillation parameters are directly related to biological test, owed to an empirically extracted cell-electrode electrical model. A discrete prototype is proposed and experimental results with living cell culture are presented, achieving the expected performances.

Keywords

bioelectric phenomena; biomedical electrodes; biomedical electronics; cellular biophysics; electric impedance; electric sensing devices; oscillators; patient monitoring; bio-oscillator; biological oscillator; cell-culture assays; cell-culture real-time monitoring; cell-electrode electrical model; oscillation-based approach; Biomedical measurement; Electrodes; Frequency measurement; Impedance; Integrated circuit modeling; Oscillators; Sensors; Bioimpedance sensors; Electrical Cell-substrate Impedance Spectroscopy (ECIS); electrical cell-substrate impedance spectroscopy (ECIS); microelectrode;

fLanguage

English

Journal_Title

Circuits and Systems II: Express Briefs, IEEE Transactions on

Publisher

ieee

ISSN

1549-7747

Type

jour

DOI

10.1109/TCSII.2014.2386233

Filename

6998822