DocumentCode :
1117704
Title :
Phage-Based Magnetoelastic Wireless Biosensors for Detecting Bacillus Anthracis Spores
Author :
Wan, Jiehui ; Shu, Huihua ; Huang, Shichu ; Fiebor, Ben ; Chen, I-Hsuan ; Petrenko, Valery A. ; Chin, Bryan A.
Author_Institution :
Dept. of Mater. Eng., Auburn Univ., AL
Volume :
7
Issue :
3
fYear :
2007
fDate :
3/1/2007 12:00:00 AM
Firstpage :
470
Lastpage :
477
Abstract :
A biosensor for the detection of biological warfare agents (Bacillus anthracis spores) was developed that combines the phage display technique with a magnetoelastic wireless detection platform. The affinity-based biosensor utilizes a phage-derived diagnostic probe as the biomolecular recognition element to capture target agents multivalently. Upon binding of the target agent to the sensor surface, the resonance frequency of the magnetoelastic biosensors decreases due to the additional mass of the target agent. Scanning electron microscopy was used to confirm binding of spores to the sensor surface. The sensitivity of the magnetoelastic acoustic sensor was tested to be 130 Hz per order of magnitude of spore concentration with a detection limit of 103 spores/ml. The specificity of the sensors was tested against spores of other closely related Bacillus species and a large preferential binding to Bacillus anthracis spores was observed. The longevity of the phage based biosensor was compared to traditional antibody based biosensors and found to exhibit a much longer life
Keywords :
biosensors; hazardous materials; magnetoelastic effects; microorganisms; military equipment; probes; scanning electron microscopy; wireless sensor networks; 130 Hz; acoustic sensor; affinity-based biosensor; bacillus anthracis spores detection; biological warfare agents; biomolecular recognition element; magnetoelastic wireless biosensors; phage display technique; phage-derived diagnostic probe; resonance frequency; scanning electron microscopy; target agents; Acoustic sensors; Acoustic testing; Biosensors; Displays; Fungi; Magnetic resonance; Magnetic sensors; Probes; Target recognition; Wireless sensor networks; Bacillus anthracis spores; magnetoelastic; phage;
fLanguage :
English
Journal_Title :
Sensors Journal, IEEE
Publisher :
ieee
ISSN :
1530-437X
Type :
jour
DOI :
10.1109/JSEN.2006.890135
Filename :
4100638
Link To Document :
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