Bayesian Image Modeling of cDNA Microarray Spots

This letter explores the potential of Bayesian signal processing for improved modeling of microarray images and enhanced estimation of gene expression ratios. Building upon our earlier work, we describe a novel elliptical spot shape model, with a Bayesian model-fitting method. The analysis of gene replicates at the image-modeling level is also briefly discussed. Prior knowledge from neighboring spots is encompassed in the framework of a Markov random field, potentially enhancing the accuracy and reliability of ratio estimates. The techniques may be particularly beneficial for irregular, overlapping, damaged, saturated, or weakly expressed spots.