Determining a Pulse Coupling to Subcellular Components With a Frequency-Domain Transfer Function

This paper describes the theoretical analysis of an external rectangular time-varying electric field pulse to subcellular components in a two-shell model using published parameters. The methods use a linear analysis that provides a fourth-order solution in the frequency domain. The fourth-order model provides increased accuracy over a lower order model to calculate responses to submicrosecond pulses and susceptibility to parameter variations. The time response of a pulsed 50-ns extracellular electrical field to a nucleoplasm provides a means of comparing cell parameter estimates