Title :
Equilibrium Binding in Immunosensors
Author :
Liu, Benjamin L. ; Schultz, Jerome S.
Author_Institution :
Medical Instrument Systems Research Division, Eli Lilly and Company
Abstract :
An in vitro solid-phase immunoassay system was studied in order to characterize immunosensor binding reactions. Several variables were explored, including the spacing of immobilized antibodies and the size and valence of analyte analogs. Under appropriate conditions, binding enhancement was observed, suggesting that the chemistry of the system can be experimentally controlled in order to meet specified criteria for sensitivity and other characteristics of the biosensor.
Keywords :
Biosensors; Bovine; Chemistry; Control systems; Immune system; In vitro; In vivo; Optical fibers; Patient monitoring; Sensor phenomena and characterization; Binding, Competitive; Blood Chemical Analysis; Fluorescence; Humans; Immunoassay; Kinetics;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
DOI :
10.1109/TBME.1986.325887
