Title :
Recovery of very low intensity CCD images from noise
Author :
Kolbe, W.F. ; Turko, B.T.
Author_Institution :
Lawrence Berkeley Lab., CA, USA
fDate :
2/1/1989 12:00:00 AM
Abstract :
A scheme for detecting low-intensity images with a CCD (charge coupled device) camera is described in which successive images are digitized at fast frame rates and averaged to enhance the signal-to-noise ratio. The results demonstrate that a dynamic range in excess of 10000:1 can be obtained using an uncooled device and averaging times of 100 s, a sensitivity comparable to that obtained with cooled devices. The technique is illustrated by imaging the weak fluorescence produced by an ethidium bromide stained DNA electrophoresis gel
Keywords :
DNA; charge-coupled devices; electrophoresis; fluorescence; gels; photoluminescence; radiation detection and measurement; CCD camera; DNA electrophoresis gel; averaging times; ethidium bromide stained; fluorescence; low intensity CCD images; noise; signal-to-noise ratio; Charge coupled devices; Charge-coupled image sensors; Computer displays; Dark current; Detectors; Light sources; Noise generators; Noise reduction; Signal to noise ratio; Temperature;
Journal_Title :
Nuclear Science, IEEE Transactions on
