Scattering-Based Cytometric Detection Using Integrated Arrayed Waveguides With Microfluidics

Author

Chen, Chun-Hao ; Tsai, Frank ; Lien, Victor ; Justis, Nicole ; Lo, Yu-Hwa

Author_Institution

Dept. of Electr. & Comput. Eng, Univ. of California San Diego, La Jolla, CA

Volume

19

Issue

6

fYear

2007

fDate

3/15/2007 12:00:00 AM

Firstpage

441

Lastpage

443

Abstract

Scattering-based on-chip excitation and detection on the arrayed-waveguide platform has been developed and implemented. Detected signals were processed with the proposed multiplication-based cross-correlation algorithm. The algorithm is capable of not only achieving signal enhancement (80 dB relative to the untreated signals) but also velocity measurement. Thus, particles flowing at different speed could also be accurately detected. Processed signals were verified by comparison to frame-by-frame video images, showing excellent correspondence. The signal processing algorithm can be easily extended to real-time on-chip detection

Keywords

biological techniques; flow measurement; integrated optics; lab-on-a-chip; light scattering; microfluidics; microsensors; optical arrays; optical correlation; optical sensors; optical waveguides; arrayed waveguides; arrayed-waveguide platform; cross-correlation algorithm; cytometric detection; frame-by-frame video images; integrated waveguides; lab-on-a-chip flow cytometry; microfluidics; multiplication-based algorithm; on-chip detection; on-chip excitation; particle flow; real-time detection; scattering-based detection; signal detection; signal enhancement; signal processing algorithm; velocity measurement; Light scattering; Microfluidics; Optical arrays; Optical coupling; Optical refraction; Optical scattering; Optical variables control; Optical waveguides; Particle scattering; Signal processing algorithms; Biosensor; flow cytometer; integrated optics; optical waveguide; scattering;

fLanguage

English

Journal_Title

Photonics Technology Letters, IEEE

Publisher

ieee

ISSN

1041-1135

Type

jour

DOI

10.1109/LPT.2007.892906

Filename

4130442