A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy

Author

Santarelli, Maria Filomena ; Sani, Lorenzo ; Ahluwalia, Arti ; Vozzi, Giovanni ; Landini, Luigi ; De Rossi, Danilo

Author_Institution

Inst. of Clinical Physiol., Italian Nat. Res. Council, Pisa, Italy

Volume

2

Issue

2

fYear

2003

fDate

6/1/2003 12:00:00 AM

Firstpage

110

Lastpage

117

Abstract

This paper presents a new image processing technique for estimating cell numbers and contours in three-dimensional (3-D) microfabricated scaffolds. The method is based on a statistical approach, and utilizes the extreme value theory, which assumes that cells in the image field are rare, high-intensity events. Confocal microscopy images of fibroblasts on 3-D structures were processed using the method, and the resulting data on cell numbers was compared with countings obtained using a Burker chamber. The results were identical to within a few percent.

Keywords

biological techniques; cellular biophysics; fluorescence; image processing; optical microscopy; Burker chamber; cell counting; cellular imaging; extreme value theory; fluorescence confocal microscopy; rare high-intensity events; statistical approach; statistical image processing; three-dimensional microfabricated scaffolds; Cells (biology); Councils; DNA; Fibroblasts; Fluorescence; Image processing; Monitoring; Optical imaging; Optical microscopy; Physiology; 3T3 Cells; Algorithms; Animals; Cell Count; Cell Culture Techniques; Cell Size; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Membranes, Artificial; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Reproducibility of Results; Sensitivity and Specificity; Tissue Engineering;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2003.813938

Filename

1209639