Title :
Tunable diode laser spectroscopy at 1.6 and 2 μm for detection of Helicobacter pylori infection using 13C-urea breath test
Author :
Sandström, Lars G. ; Lundqvist, Stefan H. ; Petterson, Anders B. ; Shumate, Michael S.
Author_Institution :
Dept. of Microelectron. ED, Chalmers Univ. of Technol., Goteborg, Sweden
Abstract :
Tunable diode laser spectroscopy has been applied to diagnosis of Helicobacter pylori by detecting the 13CO2 to isotope ratio in human breath. A number of line pairs in the 1.6- and 2.0-μm band suitable for isotope ratio detection has been investigated in terms of temperature and pressure stability of the detected isotope ratio. Two wavelength modulated diode laser absorption spectrometers (WMS), one in each band, were set up to find the detection limit in isotope abundance difference. Isotope ratio measurements have been performed on 13CO2 enriched gases with isotope ratios calibrated against an isotope ratio mass spectrometer (IRMS). The agreement between the delta values measured with the WMS technique and the IRMS was excellent. Eight breath samples from otherwise healthy volunteers were simultaneously analyzed by IRMS and the present method for possible infection with Helicobacter pylori
Keywords :
carbon compounds; diseases; isotope relative abundance; laser applications in medicine; microorganisms; patient diagnosis; 1.6 mum; 2 mum; 13C-urea breath test; 13CO2 enriched gases; CO2; Helicobacter pylori infection; breath samples; diagnosis; healthy volunteers; human breath; isotope abundance difference; isotope ratio detection; isotope ratio mass spectrometer; isotope ratio measurements; pressure stability; tunable diode laser spectroscopy; wavelength modulated diode laser absorption spectrometers; Absorption; Diode lasers; Gas detectors; Humans; Isotopes; Spectroscopy; Stability; Temperature; Tunable circuits and devices; Wavelength measurement;
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
DOI :
10.1109/2944.796328
