Title :
Two-Dimensional Materials as Substrates for the Development of Origami-Based Bionanosensors
Author :
Rahman, Masudur ; Norton, Michael L.
Author_Institution :
Dept. of Chem., Marshall Univ., Huntington, WV, USA
Abstract :
In this paper, we report the development of methods for the stable localization of DNA origami as bionanostructures for potential molecular optoelectronic sensor systems. The microcontact printing approach has been adapted to provide a rapid method for the production of self-assembled monolayer nanopatterning of aminopropyltriethoxysilane (APTES) on mica substrates. 1.2 μm blazed gratings were used as molds in the production of soft polydimethylsiloxane stamps. This stamp has been used to prepare fine-line patterns of APTES on flat mica surfaces. A solution-phase method was used to coat the bare mica between these APTES lines with dichlorodimethylsilane, which suppresses nonspecific binding of DNA origami nanostructures. Imaging studies confirm the addressing of rectangular DNA origami structures to the APTES patterns on mica.
Keywords :
DNA; biomedical materials; biosensors; molecular biophysics; monolayers; nanobiotechnology; nanopatterning; nanosensors; self-assembly; substrates; APTES; DNA; aminopropyltriethoxysilane; bionanostructures; blazed gratings; dichlorodimethylsilane; mica; microcontact printing; nanopatterning; origami-based bionanosensors; self-assembled monolayer; soft polydimethylsiloxane stamps; solution-phase method; two-dimensional materials; Atomic force microscopy; Biological materials; Chemistry; DNA; Mechanical factors; Nanostructured materials; Nanostructures; Phased arrays; Production; Rough surfaces; Substrates; Surface roughness; Surface topography; Surface treatment; APTES; DNA origami; bionanosensors; mica; microcontact print ($mu$CP);
Journal_Title :
Nanotechnology, IEEE Transactions on
DOI :
10.1109/TNANO.2010.2060494
