Combined Effect of N-Acetylcysteine (NAC) and Plasma on Proliferation of HepG2 Cells

Author

Zhao, S. ; Xiong, Z. ; Mao, X. ; Lu, X. ; He, G. ; Han, F. ; Yang, G.

Author_Institution

Key Lab. of Mol. Biophys. of Minist. of Educ., Huazhong Univ. of Sci. & Technol., Wuhan, China

Volume

40

Issue

9

fYear

2012

Firstpage

2179

Lastpage

2184

Abstract

It is found that proliferation of HepG2 cells is promoted in the N-acetylcysteine (NAC) and plasma cotreatment group while the group without NAC undergoes cell apoptosis or cell death after plasma treatment. Further investigation found that NAC and plasma cotreatment accelerates the G1 to S phase transition of HepG2 cells and cyclinD1, which responds for the proliferation, is shown to be significantly up-regulated in the NAC and plasma cotreatment group. This finding provides a rationale for developing a proliferation-promotion approach and sheds new sights on the potential application of cold plasmas such as large-scale culture of stem cells in vitro for stem cell transplantation, enhancing transplanted tissue incorporation.

Keywords

biological techniques; cellular biophysics; organic compounds; plasma applications; tissue engineering; HepG2 cells proliferation; N-acetylcysteine; NAC; cell apoptosis; cell death; cold plasmas; cyclinD1; large-scale culture; phase transition; plasma cotreatment group; plasma treatment; proliferation-promotion approach; stem cell transplantation; transplanted tissue incorporation; Acceleration; Biomembranes; In vitro; Plasmas; Proteins; Stem cells; USA Councils; Cell proliferation; N-acetylcysteine (NAC); combined effect; plasma jet;

fLanguage

English

Journal_Title

Plasma Science, IEEE Transactions on

Publisher

ieee

ISSN

0093-3813

Type

jour

DOI

10.1109/TPS.2012.2208272

Filename

6269115