Autoradiography of high-energy radionuclides using a microchannel plate detector

Author

Lees, J.E. ; Murray, A. ; Perkins, A.C. ; Fraser, G.W.

Author_Institution

Space Res. Centre, Leicester Univ., UK

Volume

49

Issue

1

fYear

2002

fDate

2/1/2002 12:00:00 AM

Firstpage

153

Lastpage

155

Abstract

We report on the use of a microchannel plate-based imaging system for autoradiography of biological samples labeled with radionuclides emitting high-energy (140-2000 keV) beta particle and gamma radiation. Samples can be imaged sufficiently rapidly (exposures 1-2 h for electrophoresis gels, ~24 h for thin tissue sections) to allow imaging of nuclides with short half-lives, such as ^99mTc. Measurement of the detector efficiencies for ^99mTc and ¹⁸⁸Re isotopes is reported. Images of a variety of samples labeled with clinically important therapeutic radionuclides ⁶⁷Cu and ¹³¹I are presented. In particular, we report preliminary images of radiolabeled anti-MUC1 mucin antibody C595 currently being developed as a therapeutic conjugate for treating bladder, breast, and ovarian tumors

Keywords

beta-ray detection; calibration; cancer; electrophoresis; gamma-ray detection; gels; position sensitive particle detectors; radiation therapy; radioactive tracers; radioisotope imaging; tumours; 1 to 2 h; 140 to 2000 keV; 24 h; ¹³¹I; ¹⁸⁸Re; ⁶⁷Cu; ⁹⁹Tc^m; C595; MCP; autoradiography; beta particle radiation; biological samples; bladder tumors; breast tumors; calibration; cancer; clinically important therapeutic radionuclides; detector efficiencies; electrophoresis gels; gamma radiation; high-energy radionuclides; microchannel plate detector; microchannel plate-based imaging system; ovarian tumors; radiolabeled antiMUC1 mucin antibody; short half-lives; therapeutic conjugate; thin tissue sections; Beta rays; Biomedical imaging; Detectors; Electrokinetics; Extraterrestrial measurements; High-resolution imaging; Isotopes; Microchannel; Optical imaging; Physics;

fLanguage

English

Journal_Title

Nuclear Science, IEEE Transactions on

Publisher

ieee

ISSN

0018-9499

Type

jour

DOI

10.1109/TNS.2002.998743

Filename

998743