Title :
Influence of 60 ns pulsed electric fields on embryonic stem cells
Author :
Akiyama, Masahiro ; Shiraishi, Eri ; Sakugawa, Takashi ; Hosseini, S.H.R. ; Akiyama, Hidenori ; Shiraki, Nobuaki ; Kume, Shoen
Author_Institution :
Grad. Sch. of Sci. & Technol., Kumamoto Univ., Kumamoto, Japan
fDate :
8/1/2011 12:00:00 AM
Abstract :
Influence of pulsed electric fields on mice embryonic stem cells was investigated. The pulsed power system used in this experiment had a pulse width of 60 ns, with an electric field varying from 10 to 30 kV/cm, a current varying from 200 to 600 amperes and a repetition rate of 1 pulse per second. Results show that the pulsed electric field suppresses ES cell proliferation, while the undifferentiated marker expression is retained during proliferation.
Keywords :
bioelectric phenomena; biological effects of fields; cellular biophysics; pulsed power technology; cell proliferation suppression; current 200 A to 600 A; mice embryonic stem cells; pulsed electric field effects; pulsed power system; time 60 ns; undifferentiated marker expression; Electric fields; Generators; Magnetic circuits; Mice; Plasmas; Stem cells; Sugar industry; ES cell; differentiation; proliferation; pulsed electric field; pulsed power;
Journal_Title :
Dielectrics and Electrical Insulation, IEEE Transactions on
DOI :
10.1109/TDEI.2011.5976104
