Simultaneous measurement of electrical activity from two colonic smooth muscle layers using a dual sucrose gap apparatus

An apparatus using the sucrose gap technique is presented. With this apparatus simultaneous measurements of contractile and intracellular electrical activity from the two smooth muscle layers of the colon are made. An L-shaped muscle preparation consisting of a leg from the circular muscle layer and a leg from the longitudinal muscle layer is used. A theoretical discussion of the device´s operation is presented. Experimental results that validate the theory are included.