Effects of Atmospheric-Pressure Plasma Jet on Pepsin Structure and Function

Author

Jijie, Roxana ; Luca, Cristina ; Pohoata, Valentin ; Topala, Ionut

Author_Institution

Fac. of Phys., Alexandru Ioan Cuza Univ., Iasi, Romania

Volume

40

Issue

11

fYear

2012

Firstpage

2980

Lastpage

2985

Abstract

The atmospheric-pressure plasma jets (APPJs) are nonthermal plasma sources studied for their high potential in many medical applications where conventional solutions failed. In this paper, we show the influence of helium APPJ on pepsin structure and function. The APPJ source is characterized using voltage-current measurements, optical emission spectroscopy, and high-speed imaging. Pepsin powder was exposed to the action of the plasma jet, and then, spectroscopic investigations were carried out to obtain information on the structural modifications. Protein function was tested using an enzymatic assay.

Keywords

enzymes; high-speed optical techniques; molecular biophysics; molecular configurations; plasma jets; powders; atmospheric-pressure plasma jet; enzymatic assay; helium APPJ source; high-speed imaging; medical applications; optical emission spectroscopy; pepsin function; pepsin powder structure; voltage-current measurements; Discharges (electric); Electron tubes; Helium; Plasma temperature; Proteins; Atmospheric-pressure plasmas; plasma diagnostics; proteins;

fLanguage

English

Journal_Title

Plasma Science, IEEE Transactions on

Publisher

ieee

ISSN

0093-3813

Type

jour

DOI

10.1109/TPS.2012.2217509

Filename

6329974