Effect of tissue-engineered chitosan-poly(vinyl alcohol) nanofibrous scaffolds on healing of burn wounds of rat skin

Author

Gholipour-Kanani, Adeleh ; Bahrami, S. Hajir ; Samadi-Kochaksaraie, A. ; Ahmadi-Tafti, H. ; Rabbani, Shahram ; Kororian, Alireza ; Erfani, Elham

Author_Institution

Textile Eng. Dept., Amirkabir Univ. of Technol., Tehran, Iran

Volume

6

Issue

4

fYear

2012

fDate

12/1/2012 12:00:00 AM

Firstpage

129

Lastpage

135

Abstract

Chitosan-poly(vinyl alcohol) (Cs:PVA) nanofibrous scaffolds were electrospun from 2:3 (wt/wt) Cs:PVA solution dissolved in 80% acetic acid. In vivo study was carried out on the dorsum skin of rat which burnt with a hot brass cylinder. The scaffolds were applied in two forms, that is, acellular (n = 6) and cell-seeded with mesenchymal stem cells (n = 6). Macroscopic measurements of wound area showed good aspect healing effect of scaffolds in comparison with control wounds specially in 15 days post operating. Pathological studies were done on the wounds to investigate the healing effects. The healing process of the wound covered with Cs/PVA nanofibrous scaffolds was much rapid compared to untreated wounds. However, the presence of stem cells on this scaffolds accelerated the wound healing process owing to their ability of collagen regeneration.

Keywords

biomedical materials; cellular biophysics; diseases; electrospinning; molecular biophysics; nanofabrication; nanofibres; nanomedicine; polymer fibres; proteins; skin; tissue engineering; wounds; PVA solution; acetic acid; burn wound healing process; cell seeding; collagen regeneration; dorsum skin; electrospinning; hot brass cylinder; macroscopic measurements; mesenchymal stem cells; pathology; rat skin; time 15 d; tissue-engineered chitosan-poly(vinyl alcohol) nanofibrous scaffolds;

fLanguage

English

Journal_Title

Nanobiotechnology, IET

Publisher

iet

ISSN

1751-8741

Type

jour

DOI

10.1049/iet-nbt.2011.0070

Filename

6353662