Title :
An implantable window system for chronic two-photon microscopy on mouse organs
Author_Institution :
WPI Immunology Frontier Res. Center, Osaka Univ., Osaka, Japan
Abstract :
Animal models were developed for chronic observation with using two-photon microscopy. To demonstrate potency of the models, T-cell and macrophage migration in inguinal lymph node, spleen, abdominal cavity and kidney were observed for two weeks on single mice. The image quality was maintained high enough during an observation period to count and trace cell population and migration.
Keywords :
biomedical optical imaging; cell motility; kidney; medical image processing; optical microscopy; two-photon processes; T-cell migration; abdominal cavity; chronic two-photon microscopy; image quality; implantable window system; inguinal lymph node; kidney; macrophage migration; mouse organs; spleen; time 2 week; Biomedical imaging; Fluorescence; Green products; Laser excitation; Laser modes; Mice; Surgery; T cell; chronic observation; homeostatic proliferation; two-photon microscopy;
Conference_Titel :
World Automation Congress (WAC), 2014
Conference_Location :
Waikoloa, HI
DOI :
10.1109/WAC.2014.6935973
