Title :
Influence of Surface Preparation Parameters on the Signal Response of an Acoustic Biosensor for the Detection of a Breast Cancer Marker
Author :
Gruhl, Friederike J. ; Länge, Kerstin
Author_Institution :
Inst. for Microstructure Technol., Karlsruhe Inst. of Technol., Eggenstein-Leopoldshafen, Germany
fDate :
6/1/2012 12:00:00 AM
Abstract :
In clinical studies, high serum levels (>;196 ng/ml) of tissue inhibitor of metalloproteinases-1 (TIMP-1) have been shown to be associated with shorter survival time for breast cancer patients. We investigated TIMP-1 assays based on surface acoustic wave biosensors. Biotinylated antibody, anti-TIMP-1, was immobilized via streptavidin allowing site-directed orientation of the antibody on the biosensor surface. Streptavidin was coupled via carbodiimide chemistry to an intermediate hydrogel layer. We found that reagent incubation times and concentrations used for streptavidin coupling had a strong effect on the signal response in a subsequent TIMP-1 assay. An optimized coupling procedure based on a lower streptavidin concentration allowed the detection of 2 ng/ml TIMP-1.
Keywords :
biochemistry; biological organs; biomedical equipment; biosensors; cancer; enzymes; gynaecology; hydrogels; molecular biophysics; surface acoustic wave sensors; TIMP-1 assays; antiTIMP-1; biosensor surface; biotinylated antibody; breast cancer marker detection; breast cancer patient; carbodiimide chemistry; intermediate hydrogel layer; metalloproteinase-1; optimized coupling procedure; serum levels; signal response; streptavidin concentration; streptavidin coupling; surface acoustic wave biosensors; surface preparation parameters; tissue inhibitor; Biosensors; Breast cancer; Couplings; Proteins; Surface acoustic waves; Surface morphology; Biosensor; cancer; surface acoustic wave; surface treatment;
Journal_Title :
Sensors Journal, IEEE
DOI :
10.1109/JSEN.2011.2175722
