Title :
High-Speed Three-Dimensional Microscope for Dynamically Moving Biological Objects Based on Parallel Phase-Shifting Digital Holographic Microscopy
Author :
Tahara, Tatsuki ; Yonesaka, Ryosuke ; Yamamoto, Seiji ; Kakue, Takashi ; Xia, Peng ; Awatsuji, Yasuhiro ; Nishio, Kenzo ; Ura, Shogo ; Kubota, Toshihiro ; Matoba, Osamu
Author_Institution :
Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan
Abstract :
We succeeded in 3-D motion-picture imaging for dynamic motion of biological objects swimming at different depths by parallel phase-shifting digital holographic microscopy (PPSDHM). PPSDHM is a technique for high-quality instantaneous 3-D imaging of specimens in the microscopic field of view by single-shot recording of multiple phase-shifted holograms. We constructed a PPSDHM for recording high-speed dynamic phenomena and then motions of biological objects in water were recorded at 20 000 and 150 000 frames/s, respectively, which is the fastest among the previous reports on 3-D imaging of biological objects.
Keywords :
biological techniques; holographic interferometry; optical microscopy; 3D motion picture imaging; PPSDHM; dynamically moving biological objects; high quality instantaneous 3D imaging; high speed 3D microscope; multiple phase shifted holograms; parallel phase shifting digital holographic microscopy; single shot recording; swimming biological objects; Biology; Holography; Image reconstruction; Image sensors; Microscopy; Three dimensional displays; Holography; holographic interferometry; holographic recording; microscopy; optical interferometry;
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
DOI :
10.1109/JSTQE.2011.2178062
