Title :
Development and calibration of a microfluidic biofilm growth cell with flow-templating and multi-modal characterization
Author :
Paquet-Mercier, Francois ; Karas, Adnane ; Safdar, Muhammad ; Aznaveh, Nahid Babaei ; Zarabadi, Mirpouyan ; Greener, Jesse
Author_Institution :
Dept. de Chim., Univ. Laval, Quebec City, QC, Canada
Abstract :
We report the development of a microfluidic flow-templating platform with multi-modal characterization for studies of biofilms and their precursor materials. A key feature is a special three inlet flow-template compartment, which confines and controls the location of biofilm growth against a template wall. Characterization compartments include Raman imaging to study the localization of the nutrient solutions, optical microscopy to quantify biofilm biomass and localization, and cyclic voltammetry for flow velocity measurements. Each compartment is tested and then utilized to make preliminary measurements.
Keywords :
bioMEMS; biomedical materials; flow measurement; microfluidics; optical microscopy; velocity measurement; voltammetry (chemical analysis); Raman imaging; biofilm biomass; biofilm localization; characterization compartments; cyclic voltammetry; flow velocity measurement; inlet flow-template compartment; microfluidic biofilm growth cell; microfluidic flow-templating platform; multimodal characterization; nutrient solutions; optical microscopy; template wall; Biomedical optical imaging; Electrodes; Microchannels; Microfluidics; Optical imaging; Optical variables measurement; Surface treatment;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
Conference_Location :
Chicago, IL
DOI :
10.1109/EMBC.2014.6943900
