DocumentCode :
140267
Title :
Magnetic resonance spectroscopy and imaging can differentiate between engineered bone and engineered cartilage
Author :
Pothirajan, Padmabharathi ; Ravindran, Sriram ; George, A. ; Magin, Richard L. ; Kotecha, Mrignayani
Author_Institution :
Dept. of Bioeng., Univ. of Illinois at Chicago, Chicago, IL, USA
fYear :
2014
fDate :
26-30 Aug. 2014
Firstpage :
3929
Lastpage :
3932
Abstract :
In the situation when both cartilage and its underlying bone are damaged, osteochondral tissue engineering is being developed to provide a solution. In such cases, the ability to non-invasively monitor and differentiate the development of both cartilage and bone tissues is important. Nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI) have been widely used to non-invasively assess tissue-engineered cartilage and tissue-engineered bone. The purpose of this work is to assess differences in MR properties of tissue-engineered bone and tissue-engineered cartilage generated from the same cell-plus-scaffold combination at the early stage of tissue growth. We developed cartilage and bone tissue constructs by seeding human marrow stromal cells (HMSCs, 2 million/ml) in 1:1 collagen/chitosan gel for four weeks. The chondrogenic or osteogenic differentiation of cells was directed with the aid of a culture medium containing chondrogenic or osteogenic growth factors, respectively. The proton and sodium NMR and waterproton T1, T2 and diffusion MRI experiments were performed on these constructs and the control collagen/chitosan gel using a 9.4 T (1H freq. = 400 MHz) and a 11.7 T (1H freq. = 500 MHz) NMR spectrometers. In all cases, the development of bone and cartilage was found to be clearly distinguishable using NMR and MRI. We conclude that MRS and MRI are powerful tools to assess growing osteochondral tissue regeneration.
Keywords :
biodiffusion; biomedical MRI; bone; cellular biophysics; gels; patient monitoring; proton magnetic resonance; tissue engineering; HMSC; MR properties; MRS; bone tissue engineering; cartilage tissue constructs; cartilage tissue engineering; cell-plus-scaffold combination; chondrogenic cell differentiation; chondrogenic growth factors; collagen-chitosan gel; damaged tissue engineering; diffusion MRI; human marrow stromal cells; magnetic resonance imaging; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; osteochondral tissue engineering; osteochondral tissue regeneration; osteogenic cell differentiation; osteogenic growth factors; proton NMR spectrometers; sodium NMR spectrometers; tissue growth; water-proton T1 MRI; water-proton T2 MRI; Bones; Coherence; Magnetic resonance imaging; Nuclear magnetic resonance; Protons; Spectroscopy; Tissue engineering;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
Conference_Location :
Chicago, IL
ISSN :
1557-170X
Type :
conf
DOI :
10.1109/EMBC.2014.6944483
Filename :
6944483
Link To Document :
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