DocumentCode :
1422865
Title :
Development of PCR Microchip for Early Cancer Risk Prediction
Author :
Jha, Sandeep Kumar ; Joo, Gi-Sung ; Ra, Gyu-Sik ; Lee, Hyun Ho ; Kim, Yong-Sang
Author_Institution :
Dept. of Nano Sci. & Eng., Myongji Univ., Yongin, South Korea
Volume :
11
Issue :
9
fYear :
2011
Firstpage :
2065
Lastpage :
2070
Abstract :
An integrated continuous-flow microfluidic chip was fabricated on glass substrate with polydimethylsiloxane (PDMS)-based microchannels, cell lysis and Polymerase chain reaction (PCR) modules on the same chip. While gold-microelectrode was used for electrochemical cell lysis, indium-tin-oxide (ITO) microheater was used for thermal cycling during PCR reaction. The fabricated device was used for PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The PCR product (193 bp) was verified for MCF10a cells by agarose gel electrophoresis after 20 cycles of reaction on the microchip, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for the entire reaction was less than 45 min. Therefore, the proposed microchip can be helpful in predicting the risk of metastatic cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.
Keywords :
DNA; bioMEMS; biochemistry; biomedical electrodes; cancer; cellular biophysics; electrochemical electrodes; electrophoresis; enzymes; gels; genetics; lab-on-a-chip; microchannel flow; microelectrodes; microfabrication; molecular biophysics; tumours; PCR amplification; PCR microchip; PCR reaction; SiO2; agarose gel electrophoresis; cancer risk prediction; electrochemical cell lysis; genetic analysis; genetic tumor human samples; glass substrate; gold-microelectrode; indium-tin-oxide microheater; integrated continuous-flow microfluidic chip; metastatic cancer; nontumorigenic MCF10a human cell lines; pancreatic cancer DNA marker; polydimethylsiloxane-based microchannels; polymerase chain reaction modules; thermal cycling; tumorigenic MCF7 cells; Cancer; DNA; Glass; Lithography; Microchannel; Substrates; Electrochemical cell lysis; SMAD4; micro heater; microfluidics; pancreatic cancer; polydimethylsiloxane (PDMS); polymerase chain reaction (PCR) microchip;
fLanguage :
English
Journal_Title :
Sensors Journal, IEEE
Publisher :
ieee
ISSN :
1530-437X
Type :
jour
DOI :
10.1109/JSEN.2011.2105262
Filename :
5685245
Link To Document :
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