Reliability Analysis of THz Characterization of Modified and Unmodified Vector Sequences

Author

Globus, Tatiana R. ; Norton, Michael L. ; Lvovska, Maryna I. ; Gregg, Derek A. ; Khromova, Tatyana B. ; Gelmont, Boris L.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Virginia, Charlottesville, VA, USA

Volume

10

Issue

3

fYear

2010

fDate

3/1/2010 12:00:00 AM

Firstpage

410

Lastpage

418

Abstract

As part of a broad study to survey and parse the fundamental spectroscopic features of double stranded DNA in the THz domain, we have performed a comparative study of a 2900 bp basic sequence, 3200 bp basic sequence with inserts, and segments of the basic sequence of 1000 and 500 bp over the range 10-25 cm^-1. Samples were generated by amplifying regions of the firefly luciferase gene modified and unmodified pGEM vector sequences using the polymerase chain reaction. Rich spectra have been obtained from each type of sample at varying concentrations. Major features appear to be relatively unaffected by concentration, over the range of concentrations studied. The effect of inserts, filtering, and length of DNA was shown to be distinguishable. One of the spectra of each length was deconvoluted into about 25 separated bands.

Keywords

DNA; reliability; terahertz spectroscopy; THz Characterization; double stranded DNA; fundamental spectroscopic features; modified vector sequences; pGEM vector sequences; polymerase chain reaction; reliability analysis; unmodified vector sequences; Biological materials; Chemistry; DNA; Filtering; Helium; Impurities; Polymers; Purification; Sequences; Spectroscopy; DNA; Deconvolution; PeakFit; THz spectroscopy; luciferase gene; pGEM vector; polymerase chain reaction (PCR);

fLanguage

English

Journal_Title

Sensors Journal, IEEE

Publisher

ieee

ISSN

1530-437X

Type

jour

DOI

10.1109/JSEN.2009.2038122

Filename

5419286