Title :
Using BODIPY dye-primer chemistry in large-scale sequencing
Author :
Muzny, Donna M. ; Metzker, Michael L. ; Bouck, John ; Gorrell, James H. ; Ding, Yan ; Maxim, Elizabeth ; Gobbs, R.A.
Author_Institution :
Baylor Coll. of Med., Houston, TX, USA
Abstract :
The issues related to integration of BODIPY dyes for large-scale sequencing, as well as analysis of their performance in our large-scale sequence assemblies, are discussed. Topics covered include: storage of BODIPY dye-primers, development of reliable reaction conditions, development of preformatted BODIPY dye-primer cocktails, development and testing of robotics applications, and quality assessment of BODIPY dye-primer reactions in production sequencing.
Keywords :
DNA; biochemistry; biological techniques; dyes; genetics; medical robotics; BODIPY dye-primer chemistry; integration; large-scale sequence assemblies; large-scale sequencing; preformatted BODIPY dye-primer cocktails; production sequencing; quality assessment; reliable reaction conditions; robotics applications; storage; Assembly; Bioinformatics; Chemistry; Cloning; DNA; Genomics; Large scale integration; Large-scale systems; Production; Sequences; Boron Compounds; Chromosome Mapping; DNA Primers; Drug Storage; Fluorescent Dyes; Humans; Quality Control; Reproducibility of Results; Robotics; Sequence Analysis, DNA;
Journal_Title :
Engineering in Medicine and Biology Magazine, IEEE
