Optical Assay for Biotechnology and Clinical Diagnosis

Author

Moczko, E. ; Cauchi, M. ; Turner, C. ; Meglinski, I. ; Piletsky, S.

Author_Institution

Cranfield Univ., Cranfield, UK

Volume

58

Issue

8

fYear

2011

Firstpage

2154

Lastpage

2160

Abstract

In this paper, we present an optical diagnostic assay consisting of a mixture of environmental-sensitive fluorescent dyes combined with multivariate data analysis for quantitative and qualitative examination of biological and clinical samples. The performance of the assay is based on the analysis of spectrum of the selected fluorescent dyes with the operational principle similar to electronic nose and electronic tongue systems. This approach has been successfully applied for monitoring of growing cell cultures and identification of gastrointestinal diseases in humans.

Keywords

bio-optics; diseases; dyes; fluorescence; patient diagnosis; biotechnology; clinical diagnosis; electronic nose; electronic tongue; environmental-sensitive fluorescent dye; gastrointestinal disease; multivariate data analysis; optical diagnostic assay; Artificial neural networks; Biomedical optical imaging; Diseases; Fluorescence; Microorganisms; Optical sensors; Principal component analysis; Chemometrics; clinical applications; fluorescence dyes; optical diagnosis; Algorithms; Biological Assay; Biomimetics; Biopolymers; Diagnosis, Computer-Assisted; Fluorescent Dyes; Gastrointestinal Diseases; Humans; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2011.2113348

Filename

5711652