Influence of the Solution on Adhesion Layer Polymerized in Radio Frequency Pulsed Plasma for DNA Hybridization Assay

Author

Chen, Qiang ; Yang, Lizhen ; Wang, Zhenduo ; Liu, Zhongwei

Author_Institution

Lab. of Plasma Phys. & Mater., Beijing Inst. of Graphic Commun., Beijing, China

Volume

38

Issue

8

fYear

2010

Firstpage

1897

Lastpage

1902

Abstract

A radio frequency (RF) pulsed plasma polymerized allylamine (PPPAA) film as an adhesion layer to adsorb DNA probe and then hybridize with a complementary or a mismatch DNA target is investigated in this paper. The possible application of plasma polymerization film as an adhesion layer for DNA hybridization assay is evaluated by DNA hybridization reactions. It was found, by surface plasmon enhanced fluorescence spectroscopy (SPFS), that the DNA hybridization reactions in phosphate buffered saline (PBS) solution can be remarkably revealed by fluorescence signals, but it heavily depends on the solution conditions. The influence of the solution pH value and the ionic strength on the PPPAA film thickness and adsorption is explored in detail.

Keywords

DNA; adhesion; adsorbed layers; fluorescence; plasma applications; polymer films; polymer solutions; polymerisation; surface plasmon resonance; DNA hybridization assay; DNA probe adsorption; PBS solution; PPPAA film; SPFS; adhesion layer; allylamine; complementary DNA target; fluorescence signals; ionic strength; mismatch DNA target; phosphate buffered saline; plasma polymerization film; radio frequency pulsed plasma; solution influence; solution pH value; surface plasmon enhanced fluorescence spectroscopy; DNA hybridization assay; fluorescence spectroscopy; plasma polymerization;

fLanguage

English

Journal_Title

Plasma Science, IEEE Transactions on

Publisher

ieee

ISSN

0093-3813

Type

jour

DOI

10.1109/TPS.2010.2044518

Filename

5454392