Title :
Specific Protein Detection in Multiprotein Coexisting Environment by Using LSPR Biosensor
Author :
Li, Shuhong ; Yin, Shaoyun ; Jiang, Yueyun ; Yin, Changcheng ; Deng, Qiling ; Du, Chunlei
Author_Institution :
State Key Lab. of Opt. Technol. for Microfabrication, Chinese Acad. of Sci., Chengdu, China
Abstract :
Specific protein detection in multiprotein coexisting environment by localized surface plasmon resonance (LSPR) biosensor is studied. After the surfaces of self-assembly fabricated triangular Ag nanoparticles on the chip are activated, a specific biological molecule layer with δ-toxic protein identifying function is linked to the chip. Based on the LSPR spectrometer measurement system in our laboratory, δ-toxic protein in sonicated Bacillus thuringiensis cell fractions (which consists of many kinds of proteins) is detected successfully by measuring the extinction spectrum of the chip.
Keywords :
biological techniques; biosensors; cellular biophysics; extinction coefficients; microorganisms; molecular biophysics; nanobiotechnology; nanoparticles; optical sensors; proteins; self-assembly; silver; surface plasmon resonance; toxicology; δ-toxic protein identifying function; Ag; LSPR biosensor; LSPR spectrometer measurement system; extinction spectrum; localized surface plasmon resonance biosensor; multiprotein coexisting environment; protein detection; self-assembly fabricated triangular nanoparticles; sonicated Bacillus thuringiensis cell fractions; specific biological molecule layer; Biomedical measurements; Biosensors; Chemical technology; Nanoparticles; Optical surface waves; Plasmons; Pollution measurement; Proteins; Resonance; Surface contamination; Biomedical monitoring; biosensor; localized surface plasmon resonance (LSPR); nanostructures;
Journal_Title :
Nanotechnology, IEEE Transactions on
DOI :
10.1109/TNANO.2010.2050698
