Impedance Sensing for Monitoring Neuronal Coverage and Comparison With Microscopy

Author

Wiertz, R.W.F. ; Rutten, W.L.C. ; Marani, E.

Author_Institution

Dept. of Electr. Eng., Univ. of Twente, Enschede, Netherlands

Volume

57

Issue

10

fYear

2010

Firstpage

2379

Lastpage

2385

Abstract

We investigated the applicability of electric impedance sensing (IS) to monitor the coverage of adhered dissociated neuronal cells on glass substrates with embedded electrodes. IS is a sensitive method for the quantification of changes in cell morphology and cell mobility, making it suitable to study aggregation kinetics. Various sizes of electrodes were compared for the real-time recording of the impedance of adhering cells, at eight frequencies (range: 5 Hz-20 kHz). The real part of the impedance showed to be most sensitive at frequencies of 10 and 20 kHz for the two largest electrodes (7850 and 125 600 μm²). Compared to simultaneous microscopic evaluation of cell coverage and cell spreading, IS shows more detail.

Keywords

adhesion; aggregation; bioelectric phenomena; biomedical electrodes; biomedical measurement; biomedical optical imaging; cell motility; electric impedance; electric impedance measurement; neurophysiology; optical microscopy; SiO₂; aggregation kinetics; cell adhesion; cell coverage; cell mobility; cell morphology; cell spreading; electric impedance sensing; electrodes; frequency 5 Hz to 20 kHz; microscopy; neuronal cells; Aggregation; electric impedance sensing (IS); neuronal cell coverage; neuronal cultures; Animals; Cell Adhesion; Cells, Cultured; Cerebral Cortex; Electric Impedance; Electrodes; Microscopy; Neurons; Rats; Signal Processing, Computer-Assisted;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2010.2055052

Filename

5497102