Title :
Integrated Photoacoustic and Fluorescence Confocal Microscopy
Author :
Wang, Yu ; Maslov, Konstantin ; Kim, Chulhong ; Hu, Song ; Wang, Lihong V.
Author_Institution :
Dept. of Biomed. Eng., Washington Univ. in St. Louis, St. Louis, MO, USA
Abstract :
We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.
Keywords :
angiocardiography; biomedical optical imaging; biomedical ultrasonics; blood vessels; fluorescence; laser applications in medicine; tumours; blood vessel; capillary level; dual-modality imaging system; fluorescence confocal microscopy; fluorescence contrasts; fluorescence lymphangiography; integrated photoacoustic microscopy; laser source; lymphatic vessel; microenvironments in vivo; micrometer resolution; objective lens; optical absorption; photoacoustic angiography; tumor angiogenesis; vasculature; Angiography; fluorescence confocal microscopy (FCM); lymphangiography; multimodal imaging; photoacoustic microscopy (PAM); Acoustics; Angiography; Animals; Ear; Lymphography; Mice; Mice, Nude; Microscopy, Confocal; Microscopy, Fluorescence; Photochemistry;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
DOI :
10.1109/TBME.2010.2059026