Title :
Nanoporous biocapsules for the encapsulation of insulinoma cells: biotransport and biocompatibility considerations
Author :
Leoni, Lara ; Desai, Tejal A.
Author_Institution :
Dept. of Bioeng., Illinois Univ., Chicago, IL, USA
Abstract :
This study investigates whether nanoporous micromachined biocapsules, with uniform membrane pore sizes of 24.5-nm, can be used to encapsulate insulin-secreting cells in vitro. This approach to cell encapsulation is based on microfabrication technology whereby immunoisolation membranes are bulk and surface micromachined to present uniform and well-controlled pore sizes as small as 10 nm, tailored surface chemistries, and precise microarchitectures. This study evaluates the behavior of insulinoma cells with micromachined membranes, the effect of matrix configurations within the biocapsule on cell behavior, as well as insulin and glucose transport through the biocapsule membranes.
Keywords :
biomedical materials; biomembrane transport; cellular biophysics; encapsulation; micromachining; porous materials; 24.5 nm; biocompatibility considerations; biotransport considerations; glucose transport; immunoisolation membranes; insulin transport; insulinoma cells encapsulation; matrix configurations; microfabrication technology; nanoporous micromachined biocapsules; precise microarchitectures; tailored surface chemistries; uniform membrane pore sizes; uniform well-controlled pore sizes; Biomembranes; Chemical technology; Chemistry; Encapsulation; Immune system; In vitro; Insulin; Microarchitecture; Nanoporous materials; Sugar; Animals; Biocompatible Materials; Biomedical Engineering; Capsules; Diffusion; Diffusion Chambers, Culture; Glucose; Insulin; Insulinoma; Islets of Langerhans Transplantation; Kinetics; Materials Testing; Mice; Pancreatic Neoplasms; Particle Size; Surface Properties; Tumor Cells, Cultured;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
