Title :
Optical projection tomography and light sheet microscopy for imaging in biological specimens a comparison study
Author :
Psycharakis, Stylianos ; Zacharopoulos, Athanasios ; Ripoll, Jorge ; Zacharakis, Giannis ; Riekher, Matthias ; Tavernarakis, Nektarios
Author_Institution :
Inst. of Electron. Struct. & Laser, Found. for Res. & Technol.-Hellas, Heraklion, Greece
Abstract :
Optical Projection Tomography and Light Sheet Microscopy have been recent revolutionary techniques for imaging in biological specimens with 3D volume rendering of high resolution. A comparison study is performed on the basic aspects of each modality and combined application on selected specimen is presented. The imaging performance characteristics of the two modalities are calculated and analyzed. Studies in model organisms such as Caenorhabditis Elegans and Parhyale, are shown and the significance of each method is discussed in relation to the specific applications they are best tailored for.
Keywords :
biomedical optical imaging; image resolution; microorganisms; optical tomography; rendering (computer graphics); 3D volume rendering; Caenorhabditis Elegans; Parhyale; biological specimen imaging; high-resolution images; imaging performance characteristics; light sheet microscopy; optical projection tomography; Biomedical optical imaging; Fluorescence; Microscopy; Optical imaging; Optical microscopy; Optimized production technology;
Conference_Titel :
Imaging Systems and Techniques (IST), 2014 IEEE International Conference on
Conference_Location :
Santorini
DOI :
10.1109/IST.2014.6958475
