Title :
Chondrogenic differentiation of adult mesenchymal stem cells and embryonic stem cells
Author :
Shu, K. ; Thatte, H. ; Spector, M.
Author_Institution :
Massachusetts Inst. of Technol., Cambridge, MA
Abstract :
Mesenchymal stem cell (MSC) contraction associated with chondrogenesis is attributed to the expression of alpha-smooth muscle actin (alpha-SMA). In this study, pluripotent embryonic carcinoma cells (ECCs) and MSCs were compared for cartilage histogensis. Both cell types expressed alpha-SMA in monolayer. However, when cultured in pellets and in 3-D scaffolds, only MSCs contracted and formed glycosaminoglycan (GAG)- and type II collagen-rich tissue. Under these culture conditions, MSCs appear to be superior over ECCs for cartilage regeneration.
Keywords :
biological tissues; cellular biophysics; molecular biophysics; proteins; tissue engineering; 3D scaffolds; adult mesenchymal stem cells; alpha-smooth muscle actin; cartilage histogensis; cartilage regeneration; chondrogenesis; chondrogenic differentiation; embryonic stem cells; glycosaminoglycan; pluripotent embryonic carcinoma cells; type II collagen-rich tissue; Bones; Cells (biology); DNA; Electrochemical machining; Embryo; Immune system; Mice; Muscles; Proteins; Stem cells;
Conference_Titel :
Bioengineering Conference, 2009 IEEE 35th Annual Northeast
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4362-8
Electronic_ISBN :
978-1-4244-4364-2
DOI :
10.1109/NEBC.2009.4967739
