Optofluidic biosensors based on color imaging

Author

Park, Jongwon ; Kim, Chang-Soo

Author_Institution

Electr. & Comput. Eng., Missouri Univ. of Sci. & Technol., Rolla, MO, USA

fYear

2010

Firstpage

7

Lastpage

10

Abstract

A color CCD (charge-coupled device) camera is used as a chemical detector to quantify fluorescence emission intensities from two kinds of optofluidic device platforms. The fluorophores responsive to oxygen are immobilized in photo-patternable hydrogel matrices. The first device is a hydrogel sensor array formed within a commercial silicone elastomer imaging chamber. The second one involves a double-chamber fluidic device composed of a permeable membrane layer and dry film photoresist layers. The results suggest that digital color imagers, in combination with a simple color separation method, can serve as the high quality chemical detectors for fluidic biosensors and biological culture platforms.

Keywords

CCD image sensors; biosensors; chemical sensors; fluidic devices; hydrogels; image colour analysis; optical sensors; photoresists; charge-coupled device; chemical detector; chemical detectors; color CCD camera; color imaging; color separation method; digital color imagers; dry film photoresist layers; fluorescence emission intensities; hydrogel sensor array; optofluidic biosensors; permeable membrane layer; photopatternable hydrogel matrices; Biomembranes; Biosensors; Charge coupled devices; Charge-coupled image sensors; Chemical and biological sensors; Color; Detectors; Fluorescence; Image sensors; Sensor arrays; CoverWell; MX5000; dry resist; polyethylene glycol (PEG); ruthenium-complex; self-calibration;

fLanguage

English

Publisher

ieee

Conference_Titel

Imaging Systems and Techniques (IST), 2010 IEEE International Conference on

Conference_Location

Thessaloniki

Print_ISBN

978-1-4244-6492-0

Type

conf

DOI

10.1109/IST.2010.5548539

Filename

5548539