• DocumentCode
    1571797
  • Title

    Inguinal skin window for chronic two-photon In vivo imaging of mouse lymph node

  • Author

    Komai, Yutaka ; Veeraveedu, Punniyakoti Thanikachalam ; Kamimura, Daisuke ; Murakami, Masaaki

  • Author_Institution
    Laboratory of Single Molecule Imaging, WPI Immunology Frontier Research Center, Osaka University, Japan
  • fYear
    2012
  • Firstpage
    1
  • Lastpage
    4
  • Abstract
    We developed an animal model for chronic observation with using two-photon microscopy. To demonstrate potency of the model, T-cell migration and proliferation in inguinal lymph node were observed for two weeks on single mice. The image quality was maintained enough high during a observation period to count and trace cell population and migration.
  • Keywords
    T cell; chronic observation; homeostatic proliferation; two-photon microscopy;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    World Automation Congress (WAC), 2012
  • Conference_Location
    Puerto Vallarta, Mexico
  • ISSN
    2154-4824
  • Print_ISBN
    978-1-4673-4497-5
  • Type

    conf

  • Filename
    6320948