Title :
Inguinal skin window for chronic two-photon In vivo imaging of mouse lymph node
Author :
Komai, Yutaka ; Veeraveedu, Punniyakoti Thanikachalam ; Kamimura, Daisuke ; Murakami, Masaaki
Author_Institution :
Laboratory of Single Molecule Imaging, WPI Immunology Frontier Research Center, Osaka University, Japan
Abstract :
We developed an animal model for chronic observation with using two-photon microscopy. To demonstrate potency of the model, T-cell migration and proliferation in inguinal lymph node were observed for two weeks on single mice. The image quality was maintained enough high during a observation period to count and trace cell population and migration.
Keywords :
T cell; chronic observation; homeostatic proliferation; two-photon microscopy;
Conference_Titel :
World Automation Congress (WAC), 2012
Conference_Location :
Puerto Vallarta, Mexico
Print_ISBN :
978-1-4673-4497-5
