Analysis of Transmembrane Potentials Induced by Pulsed Electric Field with Different Durations Based on Five-shelled Dielectric Model of Cell

In order to account for the influence of subcellular organelles on transmembrane potential, five-shelled dielectric model of spherical cell was developed in this paper. And the calculation method of transmembrane potential induced by any pulsed electric field (PEF) was put forward. Thus the effects of different pulse duration on transmembrane potentials of cell membrane and nuclear membrane were also analyzed. It is shown that there is a strong relationship between the amplitude and waveform of transmembrane potential and pulse duration. PEF with shorter duration can reach into the cell easily, thus with shorter duration pulses, transmembrane potential of nuclear membrane is increasing and close to that of cell membrane. So PEF with shorter duration will bring on some important biological effects, such as electroporation or apoptosis