Separation of blood cells by employing dielectrophoresis and traveling-wave electric fields

In this paper, we present the methods of the particle separation by employing dielectrophoresis (DEP) and traveling-wave electroosmosis (TWEO). When particles are on a plane electrode, the particles do not experience the DEP force. Because the electric field at surface of the plane electrode is zero. Thus it is necessary to transport all particles from the entire chamber to the electrode gap, where the separation of the particles can be done. To cope with this problem, we create the “Bottle neck Fork-trace electrode (BF electrode)” to separation of all particles in a chamber. The BF electrode is possible to transport all particles from the entire chamber to the electrode gap by TWEO at first, and then separate them by DEP. Leukocytes in medium are separated from erythrocytes by using this electrode.