Viscoelastic Properties of Human Mesenchymal Stem Cells

Author

Pan, Wenxiao ; Petersen, Erik ; Cai, Ning ; Ma, Gang ; Lee, Jian Run ; Feng, Zhiqin ; Liao, Kin ; Leong, Kam W.

Author_Institution

Biomedical Eng. Res. Center, Nanyang Technol. Univ.

fYear

2006

Firstpage

4854

Lastpage

4857

Abstract

In this study, we investigated the viscoelasticity of individual bone marrow-derived adult human mesenchymal stem cells (hMSCs), and the role of specific cytoskeletal component-F-actin microfilaments on the mechanical properties of individual hMSCs. The mechanical properties of hMSCs were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. For the hMSCs under control conditions the instantaneous Young´s modulus E₀ was found to be 886plusmn289(Pa), the equilibrium Young´s modulus E_infin 372plusmn125(Pa), and the apparent viscosity mu 2714plusmn1626(Pamiddots). After exposed to 2muM of chemical agent-cytochalasin D that disrupt the F-actin microfilaments, the Young´s moduli of hMSCs decreased by up to 72% and the apparent viscosity increased by 167%. These findings suggest that microfilaments are crucial in providing the viscoelastic properties of the hMSCs, and changes in the structure and properties of them may influence the mechanical properties of hMSCs significantly

Keywords

Young´s modulus; biomechanics; bone; cellular biophysics; molecular biophysics; proteins; viscoelasticity; viscosity; 247 to 497 Pa; 597 to 1175 Pa; Young modulus; apparent viscosity; bone marrow-derived adult human mesenchymal stem cells; cytochalasin D; cytoskeletal component-F-actin microfilaments; mechanical properties; micropipette aspiration technique; viscoelastic properties; viscoelasticity; Biomedical engineering; Bones; Chemical technology; Elasticity; Extracellular; Humans; Mechanical factors; Solids; Stem cells; Viscosity; Actin; Apparent viscosity; Equilibrium Young´s modulus; Instantaneous Young´s modulus; Mechanical properties; Micropipette aspiration; Viscoelasticity; human Mesenchymal Stem Cells (hMSCs);

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1615559

Filename

1615559