Title :
Evaluation method for ion transport via nanopores: Toward a neural stimulation electrode using membrane protein
Author :
Ishii, Yoshikazu ; Yagi, Tohru ; Sugawara, Michiko
Author_Institution :
Sch. of Inf. Sci. & Eng., Tokyo Inst. of Technol., Tokyo, Japan
Abstract :
We intend to develop a new stimulation electrode that contains tubular membrane proteins. A “proteoelectrode” should be capable of controlling membrane potential through the pores formed by tubular membrane proteins. We have tested the formation of lipid bilayers and the insertion of alpha-hemolysin into them. An ion indicator method for measuring molecule transfer through alpha-hemolysin in the lipid bilayer was tested. Fluorescence imaging of the ion indicator showed the assembly of a lipid bilayer and the formation of alpha-hemolysin pores. This could be a simple and effective technique for evaluating the performance of a proteoelectrode.
Keywords :
biomedical electrodes; biomembrane transport; fluorescence; lipid bilayers; molecular biophysics; proteins; proteomics; alpha-hemolysin; fluorescence imaging; ion indicator; ion transport; lipid bilayers; membrane potential; molecule transfer; nanopores; neural stimulation electrode; proteoelectrode; tubular membrane proteins; electrode; membrane protein; neural systems; stimulation;
Conference_Titel :
Biomedical Engineering International Conference (BMEiCON), 2011
Conference_Location :
Chiang Mai
Print_ISBN :
978-1-4577-2189-2
DOI :
10.1109/BMEiCon.2012.6172023
