Spectral analysis for diagnosis of rectum cancer using fluorescence and Raman spectroscopy of serum

Laser-induced fluorescence spectroscopy (LIF) and Raman spectrum of serum for diagnosis of rectum cancer were investigated by criteria of three parametric modes in this paper. The aim of this study was in an attempt to find the difference normal and rectum cancer cases, therefore 89 rectum cancer samples were measured using fluorescence and Raman spectrum. The experimental result showed that there were three obvious Raman peaks of normal case, and no Raman peak or only slight Raman peak of rectum cancer serum. Ratio of Raman peak relative intensity and fluorescence peak red shift both different. Additionally, ratio of fluorescence peak relative intensity is different at start position and end position also. And obtained 80.7% accuracy to rectum cancer compared to clinical diagnostic. It is exploring that use Raman spectrum and LIF to detection of cancer