Title :
Flow-injection photometric biosensing of cobalt(II) ions based on an apoenzyme reactivation method
Author :
Satoh, I. ; Yamada, Y.
Author_Institution :
Dept. of Chem., Technol., Kanagawa Inst. of Technol., Japan
Abstract :
Flow-injection photometric microdetermination of cobalt(II) ions based on an apoenzyme reactivation method is proposed. Alkaline phosphatase immobilized on oxirane-acrylic beads (Eupergit-C) is packed into a small polymer column and then used as the recognition element for cobalt(II) ions. The biosensing system is assembled with the immobilized enzyme column and a flow-through cell housed in a double-beam spectrophotometer for monitoring the enzymatic activity. Cobalt(II) ions are photometrically determined in 1.0 to 200 mu M through activation of the immobilized metal-free alkaline phosphatase(apoenzyme). The activity is assessed by injecting a 0.1 mL of 0.5 mM p-nitrophenyl phosphate solution as the substrate and thereby the change in absorbance at 405 nm due to the formation of p-nitrophenol resulting from the enzymic hydrolysis is measured. The column is generated by pumping a 40 mM 2,6-pyridine dicarboxylate solution (pH 6.0) between successive samples.<>
Keywords :
biological techniques and instruments; biosensors; chemical variables measurement; cobalt; photometry; proteins; 2,6-pyridine dicarboxylate solution; 405 nm; Co/sup 2+/; Eupergit-C; absorbance; alkaline phosphatase(apoenzyme); apoenzyme reactivation; biosensing; double-beam spectrophotometer; enzymatic activity; enzymic hydrolysis; flow-through cell; immobilized enzyme column; microassay; monitoring; oxirane-acrylic beads; p-nitrophenol; p-nitrophenyl phosphate solution; polymer column; pumping; Assembly systems; Biochemistry; Biosensors; Chemical technology; Copper; Monitoring; Performance evaluation; Photometry; Polymers; Zinc;
Conference_Titel :
Solid-State Sensors and Actuators, 1991. Digest of Technical Papers, TRANSDUCERS '91., 1991 International Conference on
Conference_Location :
San Francisco, CA, USA
Print_ISBN :
0-87942-585-7
DOI :
10.1109/SENSOR.1991.148978
