Nano-manipulation of single DNA molecules based on atomic force microscopy

Author

Jun-hong, LÜ ; Hai-Kuo, Li ; Hong-jie, An ; Guo-Hua, Wang ; Ying, Wang ; -Qian, Li Min ; Yi, Zhang ; Bin, Li ; Jun, Hu

Author_Institution

Shanghai Inst. of Appl. Phys., Chinese Acad. of Sci., Shanghai

fYear

2005

fDate

6/27/1905 12:00:00 AM

Firstpage

7478

Lastpage

7481

Abstract

Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10 nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed

Keywords

DNA; atomic force microscopy; biological techniques; molecular biophysics; nanobiotechnology; atomic force microscopy; cutting; dipping; folding; kneading; nanomanipulation; nanoscience; nanotechnology; picking up; positioning isolation; pushing; sequencing; single DNA molecules; Assembly; Atomic force microscopy; Atomic layer deposition; DNA; Genetic mutations; Molecular biophysics; Nanobioscience; Solids; Temperature; Tunneling; Atomic force microscopy; Molecular combing; Molecular cutting; Nano-manipulation; Single DNA molecules;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1616241

Filename

1616241