Title :
Ice Formation of Vitrification Solutions for Cryopreservation of Tissues
Author :
Liu, B.L. ; McGrath, J.J.
Author_Institution :
Inst. of Cryomedicine, Shanghai Univ. of Sci. & Technol.
Abstract :
Based on Boutron´s semi-empirical crystallization theory, a quantitative microscopic imaging method was developed to determine the quantity of ice in thin films of vitrification solutions. A universal equation was obtained for various vitrification solutions. The new method was applied to determine the ice quantity and critical cooling rates for two new vitrification solutions. This new method has several advantages including the fact that it is cheaper than differential scanning calorimetry (DSC) and that it can be used to study vitrification solutions with critical cooling rates extending beyond the maximum cooling rate of a typical DSC system
Keywords :
biological tissues; biomedical optical imaging; biothermics; cooling; crystallisation; optical microscopy; vitrification; Boutron semi-empirical crystallization theory; critical cooling rates; cryopreservation; differential scanning calorimetry; ice formation; ice quantity; quantitative microscopic imaging method; tissues; vitrification solutions; Calorimetry; Cooling; Crystallization; Equations; Glass; Ice; Inspection; Microscopy; Temperature; Vitrification; Cryopreservation; Ice Quantity; Vitrification;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616247
