Microfermentors for the rapid screening and analysis of biochemical processes

Author

Zanzotto, Andrea ; Szita, Nicolas ; Schmidt, Martin A. ; Jensen, Klavs F.

Author_Institution

Dept. of Chem. Eng., MIT, Cambridge, MA, USA

fYear

2002

fDate

6/24/1905 12:00:00 AM

Firstpage

164

Lastpage

168

Abstract

We report on a microfermentor fabricated from glass and poly(dimethylsiloxane) using techniques of soft lithography. Aeration of the bacterial culture is through a highly permeable membrane, and dissolved oxygen is monitored with the oxygen-sensitive, fluorescent compound Ru II tris(4,7-diphenyl-1,10-phenanthroline). Biomass within the microfermentor is monitored by measuring light transmission through the medium and relating this to the optical density. We present results on the fermentation of E. coli in this system

Keywords

biochemistry; biosensors; biotechnology; fermentation; microfluidics; microorganisms; microsensors; optical sensors; rapid prototyping (industrial); bacterial culture aeration; batch fermentation; biochemical processes; biocompatibility; biomass; bioprocess science; bioreactors; dissolved oxygen; fluorescence quenching; light transmission; microfermentor; optical density; optical sensing; parallel fermentation; permeable membrane; photolithography; poly(dimethylsiloxane); rapid screening; soft lithography; sterilization; Biochemical analysis; Biomass; Biomedical optical imaging; Biomembranes; Density measurement; Fluorescence; Glass; Microorganisms; Monitoring; Soft lithography;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on

Conference_Location

Madison, WI

Print_ISBN

0-7803-7480-0

Type

conf

DOI

10.1109/MMB.2002.1002306

Filename

1002306